BAIT

NAM7

IFS2, MOF4, SUP113, UPF1, ATP-dependent RNA helicase NAM7, L000002429, S000029550, L000002232, YMR080C
ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

GMC1

YDR506C
Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein
GO Process (2)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Association of yeast Upf1p with direct substrates of the NMD pathway.

Johansson MJ, He F, Spatrick P, Li C, Jacobson A

Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that detects and degrades transcripts containing premature translation termination codons. Gene expression profiling experiments have shown that inactivation of the NMD pathway leads to the accumulation of both aberrant, nonsense-containing mRNAs, and many apparently wild-type transcripts. Such increases in transcript steady-state levels could arise from direct changes in the respective mRNA half-lives, ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 26, 2007; 104(52);20872-7 [Pubmed: 18087042]

Throughput

  • High Throughput

Additional Notes

  • Transcript signal intensity, between the Upf1p-TAP and mock samples, showed a relative change of at least 2-fold. This change was reproducible in at least three of four replicate experiments and demonstrated a statistically significant P value.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NAM7 GMC1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.133BioGRID
2162104

Curated By

  • BioGRID