Publication

An improved method for the synthesis of cellulose membrane-bound peptides with free C termini is useful for PDZ domain binding studies.

Boisguerin P, Leben R, Ay B, Radziwill G, Moelling K, Dong L, Volkmer-Engert R

SPOT synthesis permits parallel synthesis and screening of thousands of cellulose membrane-bound peptides to study protein-protein interactions in a proteomic context. Recognition of C-terminal residues is one of the most common binding features of PDZ domains. Unfortunately, most solid support-bound peptide libraries lack a free C terminus due to C-terminal fixation on the solid support. To overcome this restriction, we ... [more]

Throughput

• Low Throughput

Curated By

• BioGRID