A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

A yeast model of FUS/TLS-dependent cytotoxicity.

Ju S, Tardiff DF, Han H, Divya K, Zhong Q, Maquat LE, Bosco DA, Hayward LJ, Brown RH, Lindquist S, Ringe D, Petsko GA

FUS/TLS is a nucleic acid binding protein that, when mutated, can cause a subset of familial amyotrophic lateral sclerosis (fALS). Although FUS/TLS is normally located predominantly in the nucleus, the pathogenic mutant forms of FUS/TLS traffic to, and form inclusions in, the cytoplasm of affected spinal motor neurons or glia. Here we report a yeast model of human FUS/TLS expression ... [more]

PLoS Biol. Apr. 01, 2011; 9(4);e1001052 [Pubmed: 21541368]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Additional Notes

Increased dosage of human Upf1 rescues toxicity of human FUS expression in yeast

Curated By

BioGRID

Interaction Summary

FUS

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

UPF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [IDA]RNA binding [NAS]chromatin binding [IDA]helicase activity [NAS]poly(A) RNA binding [IDA]protein binding [IPI]