An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The inner nuclear membrane protein LAP1 forms a native complex with B-type lamins and partitions with spindle-associated mitotic vesicles.

Maison C, Pyrpasopoulou A, Theodoropoulos PA, Georgatos SD

We have examined the in situ organization and nearest neighbours of the 'lamina-associated polypeptide-1' (LAP1), a type II membrane protein and a major constituent of the mammalian nuclear envelope. We show here that, during interphase, LAP1 forms multimeric assemblies which are suspended in the inner nuclear membrane and are specifically associated with B-type lamins. The LAP1-lamin B complex is distinct ... [more]

EMBO J. Aug. 15, 1997; 16(16);4839-50 [Pubmed: 9305626]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TMPO

Gene Ontology Biological Process

Gene Ontology Molecular Function

lamin binding [TAS]

Gene Ontology Cellular Component

LMNB1