BAIT

ESA1

TAS1, NuA4 histone acetyltransferase complex catalytic subunit ESA1, KAT5, L000003952, YOR244W
Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases
Saccharomyces cerevisiae (S288c)
PREY

CCT8

L000002764, YJL008C
Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.

Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Cote J, Figeys DA, Baetz K

Recent global proteomic and genomic studies have determined that lysine acetylation is a highly abundant posttranslational modification. The next challenge is connecting lysine acetyltransferases (KATs) to their cellular targets. We hypothesize that proteins that physically interact with KATs may not only predict the cellular function of the KATs but may be acetylation targets. We have developed a mass spectrometry-based method ... [more]

Proc. Natl. Acad. Sci. U.S.A. Apr. 23, 2013; 110(17);E1641-50 [Pubmed: 23572591]

Throughput

  • High Throughput

Additional Notes

  • identified by mChIP-KAT-MS assay, which consists of three steps: isolation of a lysine acetyltransferase (KAT) and its associated protein network from cells, enrichment of acetylated lysine residues within the network by an in vitro KAT reaction; and identification of the interacting proteins and acetylation sites by LC-MS/MS

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CCT8 ESA1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2331BioGRID
1938469

Curated By

  • BioGRID