BAIT

AMT1;1

ARABIDOPSIS THALIANA AMMONIUM TRANSPORT 1, ATAMT1, ATAMT1;1, T6G15.60, T6G15_60, ammonium transporter 1;1, AT4G13510
ammonium transporter 1;1
Arabidopsis thaliana (Columbia)
PREY

AMT1;3

AMMONIUM TRANSPORTER 1;3, ATAMT1;3, AT3G24300
ammonium transporter 1;3
GO Process (5)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Allosteric Regulation of Transport Activity by Heterotrimerization of Arabidopsis Ammonium Transporter Complexes in Vivo.

Yuan L, Gu R, Xuan Y, Smith-Valle E, Loque D, Frommer WB, von Wiren N

Ammonium acquisition by plant roots is mediated by AMMONIUM TRANSPORTERs (AMTs), ubiquitous membrane proteins with essential roles in nitrogen nutrition in all organisms. In microbial and plant cells, ammonium transport activity is controlled by ammonium-triggered feedback inhibition to prevent cellular ammonium toxicity. Data from heterologous expression in yeast indicate that oligomerization of plant AMTs is critical for allosteric regulation of ... [more]

Plant Cell Mar. 05, 2013; 0(0); [Pubmed: 23463773]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AMT1;3 AMT1;1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
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Curated By

  • BioGRID