SLC12A2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
COMMD1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
COMMD1 interacts with the C-terminus of NKCC1 in Calu-3 airway epithelial cells to modulate NKCC1 ubiquination.
Mice deficient in NKCC1 have been generated by targeted disruption of the gene encoding NKCC1 involving the carboxyl terminus (CT-NKCC1), but not the amino terminus. We hypothesize that resulting physiological defects are due to loss of proteins interacting with CT-NKCC1. Using a yeast two hybrid approach, adaptor protein COMMD1 was found to bind to CT-NKCC1 (aa 1040-1212). Binding was verified ... [more]
Throughput
- Low Throughput
Additional Notes
- C-terminal 173 residues of NKCC1 used as bait
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SLC12A2 COMMD1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID