Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

COMMD1 interacts with the C-terminus of NKCC1 in Calu-3 airway epithelial cells to modulate NKCC1 ubiquination.

Smith L, Litman P, Liedtke CM

Mice deficient in NKCC1 have been generated by targeted disruption of the gene encoding NKCC1 involving the carboxyl terminus (CT-NKCC1), but not the amino terminus. We hypothesize that resulting physiological defects are due to loss of proteins interacting with CT-NKCC1. Using a yeast two hybrid approach, adaptor protein COMMD1 was found to bind to CT-NKCC1 (aa 1040-1212). Binding was verified ... [more]

Am. J. Physiol., Cell Physiol. Mar. 20, 2013; 0(0); [Pubmed: 23515529]

Throughput

Low Throughput

Additional Notes

C-terminal 173 residues of NKCC1 used as bait

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SLC12A2 COMMD1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Smith L (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SLC12A2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ammonium transmembrane transporter activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

COMMD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

copper ion binding [IDA]identical protein binding [IPI]protein binding [IPI]protein homodimerization activity [IPI]