BAIT

ECM21

ART2, L000003896, YBL101C
Protein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication
UPS Project
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

LDB19

ART1, YOR322C
Alpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane
UPS Project
GO Process (2)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The ART-Rsp5 ubiquitin ligase network comprises a plasma membrane quality control system that protects yeast cells from proteotoxic stress.

Zhao Y, Macgurn JA, Liu M, Emr S

Secretory cargo that cannot fold properly in the ER are selectively targeted for removal by a well-studied ER-associated degradation pathway, or ERAD. In contrast, very little is known about post-ER quality control mechanisms for damaged or misfolded integral membrane proteins. Here we describe a quality control function of the Rsp5-ART ubiquitin ligase adaptor network that functions to protect plasma membrane ... [more]

Elife Apr. 20, 2013; 2(0);e00459 [Pubmed: 23599894]

Throughput

  • Low Throughput

Ontology Terms

  • inviable (APO:0000112)
  • temperature sensitive growth (APO:0000092)

Additional Notes

  • Although Dart2 mutant cells did not exhibit any temperature sensitive phenotypes, we found that deletion of ART2 in Dart1 mutant cells resulted in an enhanced temperature sensitivity phenotype (Figure 4D) that correlated with increased Lyp1 accumulation in the Dart1Dart2 double mutant (Figure 4C).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LDB19 ECM21
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
3394320

Curated By

  • BioGRID