CEACAM1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CARTPT
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISS]
- activation of MAPKK activity [ISS]
- adult feeding behavior [ISS]
- cell-cell signaling [TAS]
- cellular glucose homeostasis [IDA]
- cellular response to starvation [ISS]
- circadian regulation of gene expression [ISS]
- negative regulation of appetite [ISS, TAS]
- negative regulation of bone resorption [IMP]
- negative regulation of osteoclast differentiation [TAS]
- positive regulation of blood pressure [IDA]
- positive regulation of epinephrine secretion [IDA]
- positive regulation of transmission of nerve impulse [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
A secreted protein microarray platform for extracellular protein interaction discovery.
Characterization of the extracellular protein interactome has lagged far behind that of intracellular proteins, where mass spectrometry and yeast two-hybrid technologies have excelled. Improved methods for identifying receptor-ligand and extracellular matrix protein interactions will greatly accelerate biological discovery in cell signaling and cellular communication. These technologies must be able to identify low-affinity binding events that are often observed between membrane-bound ... [more]
Throughput
- High Throughput
Additional Notes
- protein microarray assay in which receptor protein was screened for interactions with a secreted protein library
Curated By
- BioGRID