Publication

A secreted protein microarray platform for extracellular protein interaction discovery.

Ramani SR, Tom I, Lewin-Koh N, Wranik B, Depalatis L, Zhang J, Eaton D, Gonzalez LC

Characterization of the extracellular protein interactome has lagged far behind that of intracellular proteins, where mass spectrometry and yeast two-hybrid technologies have excelled. Improved methods for identifying receptor-ligand and extracellular matrix protein interactions will greatly accelerate biological discovery in cell signaling and cellular communication. These technologies must be able to identify low-affinity binding events that are often observed between membrane-bound ... [more]

Throughput

• High Throughput

Additional Notes

• protein microarray assay in which receptor protein was screened for interactions with a secreted protein library

Curated By

• BioGRID