PVRL3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PVRL2
Gene Ontology Biological Process
- adherens junction organization [TAS]
- adhesion of symbiont to host [IDA]
- cell junction assembly [TAS]
- cell-cell junction organization [TAS]
- coreceptor-mediated virion attachment to host cell [IDA]
- fusion of virus membrane with host plasma membrane [IDA]
- homophilic cell adhesion via plasma membrane adhesion molecules [IDA]
- positive regulation of immunoglobulin mediated immune response [IMP]
- positive regulation of mast cell activation [IMP]
- positive regulation of natural killer cell mediated cytotoxicity [IMP]
- positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target [IMP]
- regulation of immune response [TAS]
- signal transduction [TAS]
- spermatid development [ISS]
- susceptibility to T cell mediated cytotoxicity [IDA]
- susceptibility to natural killer cell mediated cytotoxicity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
A secreted protein microarray platform for extracellular protein interaction discovery.
Characterization of the extracellular protein interactome has lagged far behind that of intracellular proteins, where mass spectrometry and yeast two-hybrid technologies have excelled. Improved methods for identifying receptor-ligand and extracellular matrix protein interactions will greatly accelerate biological discovery in cell signaling and cellular communication. These technologies must be able to identify low-affinity binding events that are often observed between membrane-bound ... [more]
Throughput
- High Throughput
Additional Notes
- protein microarray assay in which receptor protein was screened for interactions with a secreted protein library
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PVRL3 PVRL2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9986 | BioGRID | 1187758 | |
| PVRL3 PVRL2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9944 | BioGRID | 2238528 | |
| PVRL3 PVRL2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9899 | BioGRID | 3128692 | |
| PVRL2 PVRL3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | High | - | BioGRID | 858410 |
Curated By
- BioGRID