POL32
Gene Ontology Biological Process
- DNA amplification [IMP]
- DNA replication, removal of RNA primer [IDA]
- RNA-dependent DNA replication [IDA]
- base-excision repair [TAS]
- double-strand break repair via break-induced replication [IMP]
- lagging strand elongation [TAS]
- leading strand elongation [TAS]
- mismatch repair [NAS]
- nucleotide-excision repair [TAS]
- postreplication repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MMS2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Synthetic Rescue
A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.
Publication
Noncanonical role of the 9-1-1 clamp in the error-free DNA damage tolerance pathway.
Damaged DNA is an obstacle during DNA replication and a cause of genome instability and cancer. To bypass this problem, eukaryotes activate DNA damage tolerance (DDT) pathways that involve ubiquitylation of the DNA polymerase clamp proliferating cell nuclear antigen (PCNA). Monoubiquitylation of PCNA mediates an error-prone pathway by recruiting translesion polymerases, whereas polyubiquitylation activates an error-free pathway that utilizes undamaged ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- growth defect of pol32 sgs1 double mutants is rescued by mutation in MMS2
- synthetic lethality of rad9 pol32 double mutant is rescued by further deletion of MMS2, the triple mutant is alive
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POL32 MMS2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -8.4457 | BioGRID | 214844 | |
| MMS2 POL32 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1343 | BioGRID | 2115328 | |
| POL32 MMS2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1694 | BioGRID | 2138700 | |
| MMS2 POL32 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 3545707 | |
| POL32 MMS2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 447878 | |
| POL32 MMS2 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 268869 | |
| MMS2 POL32 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 159768 | |
| POL32 MMS2 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 447883 |
Curated By
- BioGRID