Microtubule plus-end proteins CLIP-170 and EB1 dynamically track the tips of growing microtubules in vivo. Here we examine the association of these proteins with microtubules in vitro. CLIP-170 binds tubulin dimers and co-assembles into growing microtubules. EB1 binds tubulin dimers more weakly, so no co-assembly is observed. However, EB1 binds to CLIP-170, and forms a co-complex with CLIP-170 and tubulin ... [more]

FEBS Lett. Feb. 20, 2006; 580(5);1327-32 [Pubmed: 16455083]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TUBB

Gene Ontology Biological Process

Gene Ontology Molecular Function

MHC class I protein binding [IDA]protein binding [IPI]structural molecule activity [TAS]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

CLIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

metal ion binding [NAS]microtubule binding [IDA]microtubule plus-end binding [IDA]protein binding [IPI]protein homodimerization activity [TAS]tubulin binding [IDA]zinc ion binding [IDA]

Gene Ontology Cellular Component

Reconstituted Complex

Publication

Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes.

Throughput

Curated By

MHC class I protein binding [IDA]
protein binding [IPI]
structural molecule activity [TAS]
ubiquitin protein ligase binding [IPI]

metal ion binding [NAS]
microtubule binding [IDA]
microtubule plus-end binding [IDA]
protein binding [IPI]
protein homodimerization activity [TAS]
tubulin binding [IDA]
zinc ion binding [IDA]