FBXL3
Gene Ontology Biological Process
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA, ISO]
- entrainment of circadian clock by photoperiod [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein destabilization [IMP]
- protein ubiquitination [IDA]
- regulation of circadian rhythm [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CRY2
Gene Ontology Biological Process
- circadian regulation of gene expression [IGI, IMP]
- circadian rhythm [IMP]
- entrainment of circadian clock by photoperiod [IMP]
- glucose homeostasis [IGI]
- lipid storage [IGI]
- negative regulation of circadian rhythm [IDA]
- negative regulation of glucocorticoid receptor signaling pathway [IGI]
- negative regulation of glucocorticoid secretion [IGI]
- negative regulation of phosphoprotein phosphatase activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [IDA, IGI, ISO]
- protein import into nucleus [IPI]
- regulation of circadian rhythm [IMP]
- response to insulin [IGI]
Gene Ontology Molecular Function- DNA binding [ISO]
- FAD binding [IDA]
- damaged DNA binding [ISO]
- kinase binding [IPI]
- nuclear hormone receptor binding [IPI]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- single-stranded DNA binding [ISO]
- transcription factor binding transcription factor activity [ISO]
- transcription regulatory region sequence-specific DNA binding [IDA]
- ubiquitin binding [ISO]
- DNA binding [ISO]
- FAD binding [IDA]
- damaged DNA binding [ISO]
- kinase binding [IPI]
- nuclear hormone receptor binding [IPI]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- single-stranded DNA binding [ISO]
- transcription factor binding transcription factor activity [ISO]
- transcription regulatory region sequence-specific DNA binding [IDA]
- ubiquitin binding [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SCF(FBXL3) ubiquitin ligase targets cryptochromes at their cofactor pocket.
The cryptochrome (CRY) flavoproteins act as blue-light receptors in plants and insects, but perform light-independent functions at the core of the mammalian circadian clock. To drive clock oscillations, mammalian CRYs associate with the Period proteins (PERs) and together inhibit the transcription of their own genes. The SCF(FBXL3) ubiquitin ligase complex controls this negative feedback loop by promoting CRY ubiquitination and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FBXL3 CRY2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| FBXL3 CRY2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID