BAIT

EOL2

ETO1-like 2, MZN1.1, AT5G58550
protein ETO1-like 2
GO Process (1)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)
PREY

ACS5

1-AMINO-1-CYCLOPROPANECARBOXYLATE SYNTHASE, ACC synthase 5, ATACS5, CIN5, CYTOKININ-INSENSITIVE 5, ETHYLENE OVERPRODUCER 2, ETO2, MPA24.15, MPA24_15, AT5G65800
1-aminocyclopropane-1-carboxylate synthase 5
GO Process (4)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

14-3-3 Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Protein Turnover in Arabidopsis.

Yoon GM, Kieber JJ

14-3-3 proteins are a family of conserved phospho-specific binding proteins involved in diverse physiological processes. Plants have large 14-3-3 gene families, and many binding partners have been identified, though relatively few functions have been defined. Here, we demonstrate that 14-3-3 proteins interact with multiple 1-aminocyclopropane-1-carboxylate synthase (ACS) isoforms in Arabidopsis thaliana. ACS catalyzes the generally rate-limiting step in the biosynthesis ... [more]

Plant Cell Mar. 19, 2013; 0(0); [Pubmed: 23512855]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EOL2 ACS5
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID