BAIT
CUL1
pcu1, SPAC17G6.12
cullin 1
GO Process (4)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
PREY
POF11
SPAC30.05, mug156, SPAC29E6.01
F-box protein Pof11
GO Process (1)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
CAND1 controls in vivo dynamics of the cullin 1-RING ubiquitin ligase repertoire.
The combinatorial architecture of cullin 1-RING ubiquitin ligases, in which multiple F-box containing substrate receptors compete for access to CUL1, poses special challenges to assembling cullin 1-RING ubiquitin ligase complexes through high affinity protein interactions while maintaining the flexibility to dynamically sample the entire F-box containing substrate receptor repertoire. Here, using highly quantitative mass spectrometry, we demonstrate that this problem ... [more]
Nat Commun Mar. 29, 2013; 4(0);1642 [Pubmed: 23535663]
Throughput
- Low Throughput
Curated By
- BioGRID