MLC1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- basolateral plasma membrane [IDA]
- caveola [ISS]
- clathrin-coated vesicle [ISS]
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- early endosome [ISS]
- endoplasmic reticulum [IDA]
- endosome [IDA]
- integral component of membrane [IDA]
- lysosome [ISS]
- membrane raft [ISS]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- recycling endosome [ISS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
MLC1 trafficking and membrane expression in astrocytes: role of caveolin-1 and phosphorylation.
Megalencephalic leukoencephalopathy with subcortical cysts (MLC) is a rare congenital leukodystrophy caused by mutations in the MLC1 gene that encodes a membrane protein of unknown function. In the brain MLC1 protein is mainly expressed in astrocyte end-feet, localizes in lipid rafts and associates with the dystrophin glycoprotein complex (DGC). Using pull-down and co-fractionation assays in cultured human and rat astrocytes, ... [more]
Throughput
- Low Throughput
Ontology Terms
- cell line: u-251 mg cell (BTO:0002035)
- disease: astrocytoma (DOID:3069)
Curated By
- BioGRID