BAIT

YIPF3

C6orf109, KLIP1, LRRGT00110, RGD1308185
Yip1 domain family, member 3
GO Process (0)
GO Function (0)
GO Component (4)

Gene Ontology Cellular Component

Rattus norvegicus
PREY

YIPF4

RGD1310157
Yip1 domain family, member 4
GO Process (0)
GO Function (0)
GO Component (4)

Gene Ontology Cellular Component

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Characterization of YIPF3 and YIPF4, cis-Golgi Localizing Yip domain family proteins.

Tanimoto K, Suzuki K, Jokitalo E, Sakai N, Sakaguchi T, Tamura D, Fujii G, Aoki K, Takada S, Ishida R, Tanabe M, Itoh H, Yoneda Y, Sohda M, Misumi Y, Nakamura N

The Yip1 domain family (YIPF) proteins are homologues of yeast Yip1p and Yif1p, which are proposed to function in ER to Golgi transport. Here, we report the characterization of YIPF3 and YIPF4, homologues of human Yif1p and Yip1p, respectively. Immunofluorescence and immuno-electron microscopy showed that both YIPF3 and YIPF4 are clearly concentrated in the cis-Golgi. While YIPF4 was detected as ... [more]

Cell Struct. Funct. Jul. 16, 2011; 36(2);171-85 [Pubmed: 21757827]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YIPF4 YIPF3
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID