MYOD1
Gene Ontology Biological Process
- cardiac muscle cell differentiation [NAS]
- cellular response to estradiol stimulus [IDA]
- cellular response to glucocorticoid stimulus [IDA]
- cellular response to oxygen levels [IDA]
- cellular response to starvation [IDA]
- histone H3 acetylation [IDA]
- histone H4 acetylation [IDA]
- muscle organ development [IDA, IGI]
- myoblast differentiation [IDA]
- myoblast fate determination [IDA]
- myoblast fusion [IMP]
- myotube cell development [ISO]
- myotube differentiation [IDA]
- myotube differentiation involved in skeletal muscle regeneration [IMP]
- negative regulation of myoblast proliferation [IMP]
- positive regulation of muscle cell differentiation [ISO]
- positive regulation of myoblast fusion [ISO]
- positive regulation of skeletal muscle tissue regeneration [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of RNA splicing [ISO]
- regulation of alternative mRNA splicing, via spliceosome [IDA, IMP]
- regulation of transcription from RNA polymerase II promoter [IDA, TAS]
- skeletal muscle cell differentiation [IMP, NAS]
- skeletal muscle fiber adaptation [IMP]
- skeletal muscle fiber development [IMP]
- skeletal muscle tissue development [IDA, IGI, IMP, TAS]
- striated muscle cell differentiation [IMP]
- transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [IDA]
- enzyme binding [IPI]
- nuclear hormone receptor binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [IDA]
- enzyme binding [IPI]
- nuclear hormone receptor binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
MAP2K1
Gene Ontology Biological Process
- Golgi inheritance [ISO]
- MAPK cascade [IMP, ISO]
- activation of MAPK activity [IMP, ISO, TAS]
- cell cycle arrest [ISO]
- cell motility [IMP]
- cell proliferation [ISO]
- cellular senescence [ISO]
- keratinocyte differentiation [IMP]
- labyrinthine layer development [IMP]
- melanosome transport [ISO]
- mitotic nuclear division [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of homotypic cell-cell adhesion [ISO]
- neuron differentiation [IMP, ISO]
- neuron projection morphogenesis [ISO]
- peptidyl-tyrosine dephosphorylation [IMP]
- placenta blood vessel development [IMP]
- positive regulation of Ras GTPase activity [ISO]
- positive regulation of Ras protein signal transduction [ISO]
- positive regulation of cell differentiation [IDA]
- positive regulation of cell migration [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of transcription elongation from RNA polymerase II promoter [ISO]
- protein heterooligomerization [ISO]
- protein phosphorylation [IMP, ISO, TAS]
- regulation of Golgi inheritance [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of stress-activated MAPK cascade [TAS]
- regulation of vascular smooth muscle contraction [ISO]
- response to axon injury [ISO]
- response to glucocorticoid [ISO]
- response to oxidative stress [ISO]
- vesicle transport along microtubule [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- MAP kinase kinase activity [IMP, ISO]
- Ras GTPase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- protein serine/threonine/tyrosine kinase activity [TAS]
- receptor signaling protein tyrosine phosphatase activity [IMP]
- ATP binding [ISO]
- MAP kinase kinase activity [IMP, ISO]
- Ras GTPase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- protein serine/threonine/tyrosine kinase activity [TAS]
- receptor signaling protein tyrosine phosphatase activity [IMP]
Gene Ontology Cellular Component
- Golgi apparatus [ISO, TAS]
- axon [ISO]
- cell cortex [ISO]
- cytoplasm [ISO]
- cytosol [ISO, TAS]
- dendrite [ISO]
- dendrite cytoplasm [ISO]
- early endosome [TAS]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule [ISO]
- mitochondrion [TAS]
- nucleus [TAS]
- perikaryon [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mitogen-activated protein kinase kinase 1 (MEK1) stabilizes MyoD through direct phosphorylation at tyrosine 156 during myogenic differentiation.
Previously, we reported that mitogen-activated protein kinase kinase 1 (MEK1) activated in the mid-stage of skeletal muscle differentiation promotes myogenic differentiation. To elucidate the molecular mechanism, we investigated an activity of MEK1 for MyoD. Activated MEK1 associates with MyoD in the nucleus of differentiating myoblasts. In vitro kinase assay using active MEK1, a (32)P-labeled protein band corresponding to GST-MyoD was ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MAP2K1 MYOD1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 860141 |
Curated By
- BioGRID