BAIT

SP1

1110003E12Rik, AA450830, AI845540, Sp1-1
trans-acting transcription factor 1
GO Process (18)
GO Function (20)
GO Component (4)

Gene Ontology Cellular Component

Mus musculus
PREY

MSX3

AI323377
msh homeobox 3
GO Process (3)
GO Function (1)
GO Component (1)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The mechanism of biogenesis and potential function of the two alternatively spliced mRNAs encoded by the murine Msx3 gene.

Matsui H, Takahashi T, Raghow R

The homeodomain-containing Msx3 gene, the newest member of the Msx family, encodes two mRNAs, with an unknown relationship to each other. To elucidate how Msx3 gene generates the two transcripts, we cloned their corresponding cDNAs from an E10.5 mouse embryo cDNA library. The alignment of sequences of the two Msx3-specific cDNAs with the corresponding regions of the genomic DNA revealed ... [more]

Mol. Cell. Biochem. Dec. 01, 2004; 267(1);1-11 [Pubmed: 15663180]

Throughput

  • Low Throughput

Curated By

  • BioGRID