Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyclosome substrate.

Emanuele MJ, Ciccia A, Elia AE, Elledge SJ

The anaphase-promoting complex/cyclosome (APC/C) is a cell cycle-regulated E3 ubiquitin ligase that controls the degradation of substrate proteins at mitotic exit and throughout the G1 phase. We have identified an APC/C substrate and cell cycle-regulated protein, KIAA0101/PAF15. PAF15 protein levels peak in the G2/M phase of the cell cycle and drop rapidly at mitotic exit in an APC/C- and KEN-box-dependent ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jun. 14, 2011; 108(24);9845-50 [Pubmed: 21628590]

Throughput

  • High Throughput

Additional Notes

  • High Throughput: The author provided a high confidence data set of PAF15 interacting proteins that were identified using the COMPASS algorithm (Sowa et al, Cell 2009).

Curated By

  • BioGRID