KCNIP3
Gene Ontology Biological Process
Gene Ontology Molecular Function
CLN3
Gene Ontology Biological Process
- action potential [ISS]
- amyloid precursor protein catabolic process [IDA]
- arginine transport [IDA]
- associative learning [ISS]
- autophagic vacuole fusion [ISS]
- cellular amino acid metabolic process [ISS]
- ceramide metabolic process [IDA]
- cytosolic calcium ion homeostasis [ISS]
- galactosylceramide metabolic process [IMP]
- globoside metabolic process [IMP]
- glucosylceramide metabolic process [IMP]
- ionotropic glutamate receptor signaling pathway [ISS]
- lysosomal lumen acidification [IMP]
- lysosomal lumen pH elevation [IDA]
- lysosome organization [ISS]
- macroautophagy [NAS]
- membrane organization [ISS]
- negative regulation of apoptotic process [IMP]
- negative regulation of catalytic activity [ISS]
- negative regulation of macroautophagy [ISS]
- negative regulation of neuron apoptotic process [ISS]
- negative regulation of proteolysis [ISS]
- neuromuscular process controlling balance [ISS]
- neurotransmitter metabolic process [ISS]
- protein catabolic process [NAS]
- protein processing [ISS]
- receptor-mediated endocytosis [IMP]
- sphingomyelin metabolic process [IMP]
- vacuolar transport [IBA]
- vesicle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- Golgi membrane [IDA]
- Golgi stack [IDA]
- autophagic vacuole [ISS]
- caveola [IDA]
- cytoplasm [IDA]
- early endosome [IDA]
- endoplasmic reticulum [IDA]
- integral component of endoplasmic reticulum membrane [IDA]
- integral component of membrane [IDA]
- late endosome [IDA, ISS]
- lysosomal membrane [IDA]
- lysosome [IDA]
- membrane raft [IDA]
- mitochondrion [TAS]
- neuron projection [IDA]
- nucleus [IDA]
- plasma membrane [IDA]
- synaptic vesicle [IDA]
- trans-Golgi network [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Neuronal vulnerability of CLN3 deletion to calcium-induced cytotoxicity is mediated by calsenilin.
Calsenilin/DREAM/KChIP3, a neuronal Ca(2+)-binding protein, has multifunctions in nucleus and cytosol. Here, we identified CLN3 as a calsenilin-binding partner whose mutation or deletion is observed in Batten disease. In vitro binding and immunoprecipitation assays show that calsenilin interacts with the C-terminal region of CLN3 and the increase of Ca(2+) concentration in vitro and in cells causes significant dissociation of calsenilin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CLN3 KCNIP3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| KCNIP3 CLN3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID