BAIT

PCNA

ATLD2
proliferating cell nuclear antigen
Homo sapiens
PREY

PCN1

pcn, SPBC16D10.09
PCNA
GO Process (2)
GO Function (2)
GO Component (4)
Schizosaccharomyces pombe (972h)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A small peptide inhibitor of DNA replication defines the site of interaction between the cyclin-dependent kinase inhibitor p21WAF1 and proliferating cell nuclear antigen.

Warbrick E, Lane DP, Glover DM, Cox LS

BACKGROUND: p21WAF1 is a potent inhibitor of the cell-cycle regulatory cyclin-dependent kinases (Cdks). It acts on Cdks in the G1 and S phases of the cell cycle, and also binds to proliferating cell nuclear antigen (PCNA), blocking DNA replication in vitro. Transcription of p21WAF1 can be induced by the human tumour suppressor protein p53, suggesting that the action of p21WAF1 ... [more]

Curr. Biol. Mar. 01, 1995; 5(3);275-82 [Pubmed: 7780738]

Throughput

  • Low Throughput

Curated By

  • BioGRID