BAIT

NDEL1

2600006O07Rik, MITAP1, NUDEL, mNudel, RP23-396M19.4
nuclear distribution gene E-like homolog 1 (A. nidulans)
GO Process (24)
GO Function (6)
GO Component (13)
Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells.

Shen Y, Li N, Wu S, Zhou Y, Shan Y, Zhang Q, Ding C, Yuan Q, Zhao F, Zeng R, Zhu X

Cdc42GAP promotes inactivation of Cdc42, a small GTPase whose activation at the leading edge by guanine nucleotide exchange factors is critical for cell migration. How Cdc42GAP is regulated to ensure proper levels of active Cdc42 is poorly understood. Here we show that Nudel, a cytoplasmic dynein regulator, competes with Cdc42 for binding Cdc42GAP. Consequently, Nudel can inhibit Cdc42GAP-mediated inactivation of ... [more]

Dev. Cell Mar. 01, 2008; 14(3);342-53 [Pubmed: 18331715]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DYNC1I1 NDEL1
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.9395BioGRID
2671145

Curated By

  • BioGRID