ITCH
Gene Ontology Biological Process
- negative regulation of JNK cascade [IMP]
- negative regulation of NF-kappaB transcription factor activity [IMP]
- negative regulation of alpha-beta T cell proliferation [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of defense response to virus [IMP, ISO]
- positive regulation of T cell anergy [IMP]
- positive regulation of protein catabolic process [IDA, IMP]
- protein K29-linked ubiquitination [ISO]
- protein K48-linked ubiquitination [IMP, ISO]
- protein K63-linked ubiquitination [ISO]
- protein polyubiquitination [IDA]
- protein ubiquitination [IBA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of protein deubiquitination [IMP]
- ubiquitin-dependent protein catabolic process [IDA, ISA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NDFIP1
Gene Ontology Biological Process
- cellular iron ion homeostasis [ISO]
- negative regulation of T cell proliferation [IMP]
- negative regulation of gene expression [ISO]
- negative regulation of inflammatory response [IMP]
- negative regulation of interleukin-4 production [IMP]
- negative regulation of isotype switching to IgE isotypes [IMP]
- negative regulation of protein transport [ISO]
- negative regulation of transporter activity [ISO]
- negative regulation of type 2 immune response [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of protein catabolic process [IDA, IMP]
- positive regulation of protein ubiquitination [ISO]
- regulation of isotype switching to IgG isotypes [IMP]
- regulation of lymphocyte differentiation [IMP]
- regulation of myeloid leukocyte differentiation [IMP]
- signal transduction [ISO]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation.
Nedd4 family interacting protein-1 (Ndfip1) is a protein whose only known function is that it binds Nedd4, a HECT-type E3 ubiquitin ligase. Here we show that mice lacking Ndfip1 developed severe inflammation of the skin and lung and died prematurely. This condition was due to a defect in Ndfip1(-/-) T cells. Ndfip1(-/-) T cells were activated, and they proliferated and ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID