Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Association of a new c-Cbl related protein with the very first stages of apoptosis induction.

Corsois L, Quatannens B, Dumont P, Aumercier M, Defresne MP, Regnier DC

This study investigates the involvement of the c-cbl proto-oncogene during the first stages of the apoptotic process. We have already shown that a c-Cbl aptotosis-related protein of 90 kDa (CARP 90) is detected very rapidly in the cytoplasm as well as in the nucleus of murine thymocytes after hydrocortisone (HC) treatment. We report here that this protein appeared as well ... [more]

Cancer Detect. Prev. Jul. 10, 2002; 26(2);93-104 [Pubmed: 12102152]

Throughput

  • Low Throughput

Curated By

  • BioGRID