PDLIM7
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SH2B2
Gene Ontology Biological Process
- B-1 B cell homeostasis [IMP]
- actin cytoskeleton organization [IMP, IPI]
- antigen receptor-mediated signaling pathway [IDA]
- brown fat cell differentiation [IDA]
- cytokine-mediated signaling pathway [IDA]
- glucose homeostasis [ISA]
- insulin receptor signaling pathway [IDA]
- intracellular signal transduction [IDA, ISO]
- negative regulation of glucose import [ISA]
- nervous system development [ISO]
- positive regulation of signal transduction [IDA]
- regulation of JAK-STAT cascade [ISO]
- regulation of Ras protein signal transduction [ISO]
- regulation of immune response [IMP]
- regulation of metabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The interaction between the adaptor protein APS and Enigma is involved in actin organisation.
APS (adaptor protein with PH and SH2 domains) is an adaptor protein phosphorylated by several tyrosine kinase receptors including the insulin receptor. To identify novel binding partners of APS, we performed yeast two-hybrid screening. We identified Enigma, a PDZ and LIM domain-containing protein that was previously shown to be associated with the actin cytoskeleton. In HEK 293 cells, Enigma interacted ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID