An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Drafting the CLN3 protein interactome in SH-SY5Y human neuroblastoma cells: a label-free quantitative proteomics approach.

Scifo E, Szwajda A, Debski J, Uusi-Rauva K, Kesti T, Dadlez M, Gingras AC, Tyynelae J, Baumann MH, Jalanko A, Lalowski M

Neuronal ceroid lipofuscinoses (NCL) are the most common inherited progressive encephalopathies of childhood. One of the most prevalent forms of NCL, Juvenile neuronal ceroid lipofuscinosis (JNCL) or CLN3 disease (OMIM: 204200) is caused by mutations in the CLN3 gene on chromosome 16p12.1. Despite progress in the NCL field, the primary function of ceroid-lipofuscinosis neuronal protein 3 (CLN3) remains elusive. In ... [more]

J. Proteome Res. Mar. 07, 2013; 0(0); [Pubmed: 23464991]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

CLN5

Gene Ontology Biological Process

Gene Ontology Molecular Function

mannose binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

SLC25A1