BAIT

NPM1

B23, NPM
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
GO Process (23)
GO Function (14)
GO Component (10)
Homo sapiens
PREY

BOLA2

BOLA2A, My016
bolA family member 2
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative Study of the Interactome of PKCζ Involved in the EGF-induced Tumor Cell Chemotaxis.

Chen R, Wang Y, Liu Y, Zhang Q, Zhang X, Zhang F, Shieh CH, Yang D, Zhang N

Chemotaxis plays an important role in metastasis. In our previous studies, we reported that protein kinase C ζ (PKCζ) mediated cancer cell chemotaxis by regulating cytoskeleton rearrangement and cell adhesion. To further study the molecular mechanism of chemotaxis, mass spectrometry-based approaches were employed to investigate the interactome of PKCζ and its changes upon stimulation by epidermal growth factor (EGF). As ... [more]

J. Proteome Res. Feb. 21, 2013; 0(0); [Pubmed: 23402259]

Throughput

  • High Throughput

Curated By

  • BioGRID