SIR2
Gene Ontology Biological Process
- chromatin silencing [IMP]
- chromatin silencing at centromere [IMP]
- chromatin silencing at silent mating-type cassette [IGI, IMP]
- chromatin silencing at telomere [IMP]
- histone H3 deacetylation [IDA]
- histone H3-K9 modification [IDA]
- histone H4 deacetylation [IDA]
- histone deacetylation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IEP]
- positive regulation of heterochromatin assembly [EXP]
- protein localization to chromosome, telomeric region [IMP]
- regulation of histone H3-K9 methylation [IMP]
Gene Ontology Molecular Function- NAD+ binding [IDA]
- NAD-dependent histone deacetylase activity (H3-K9 specific) [IDA]
- NAD-dependent histone deacetylase activity (H4-K16 specific) [IDA]
- histone deacetylase activity [IDA]
- histone deacetylase activity (H3-K14 specific) [IDA]
- histone deacetylase activity (H3-K4 specific) [IDA]
- histone deacetylase activity (H3-K9 specific) [IDA]
- histone deacetylase activity (H4-K16 specific) [IDA]
- NAD+ binding [IDA]
- NAD-dependent histone deacetylase activity (H3-K9 specific) [IDA]
- NAD-dependent histone deacetylase activity (H4-K16 specific) [IDA]
- histone deacetylase activity [IDA]
- histone deacetylase activity (H3-K14 specific) [IDA]
- histone deacetylase activity (H3-K4 specific) [IDA]
- histone deacetylase activity (H3-K9 specific) [IDA]
- histone deacetylase activity (H4-K16 specific) [IDA]
Gene Ontology Cellular Component
CLR3
Gene Ontology Biological Process
- chromatin silencing at centromere [IMP]
- chromatin silencing at rDNA [IMP]
- chromatin silencing at silent mating-type cassette [IMP]
- chromatin silencing at telomere [IMP]
- histone H3 deacetylation [IMP]
- histone deacetylation [IDA, IMP]
- maintenance of chromatin silencing at silent mating-type cassette [NAS]
- negative regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- nucleosome positioning [IMP]
- regulation of histone methylation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Phenotypic Enhancement
A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance.
Epigenetically regulated heterochromatin domains govern essential cellular activities. A key feature of heterochromatin domains is the presence of hypoacetylated nucleosomes, which are methylated on lysine 9 of histone H3 (H3K9me). Here, we investigate the requirements for establishment, spreading and maintenance of heterochromatin using fission yeast centromeres as a paradigm. We show that establishment of heterochromatin on centromeric repeats is initiated ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: silencing (APO:0000046)
- phenotype: chromosome/plasmid maintenance (APO:0000143)
Additional Notes
- double mutants show a loss of heterochromatin silencing
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SIR2 CLR3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -3.2099 | BioGRID | 525629 | |
| CLR3 SIR2 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 871652 | |
| SIR2 CLR3 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 862518 |
Curated By
- BioGRID