BAIT

PRPH

NEF4, PRPH1
peripherin
GO Process (0)
GO Function (0)
GO Component (3)

Gene Ontology Cellular Component

Homo sapiens
PREY

LNX1

LNX, MPDZ, PDZRN2, UNQ574/PRO1136
ligand of numb-protein X 1, E3 ubiquitin protein ligase
UPS Project
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of human protein interaction domains using an ORFeome-based yeast two-hybrid fragment library.

Waaijers S, Koorman T, Kerver J, Boxem M

Physical interactions between proteins are essential for biological processes. Hence, there have been major efforts to elucidate the complete networks of protein-protein interactions, or 'interactomes', of various organisms. Detailed descriptions of protein interaction networks should include information on the discrete domains that mediate these interactions, yet most large-scale efforts model interactions between whole proteins only. We previously developed a yeast ... [more]

J. Proteome Res. May. 30, 2013; 0(0); [Pubmed: 23718855]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LNX1 PRPH
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2699389

Curated By

  • BioGRID