BAIT

APP

AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2
amyloid beta (A4) precursor protein
GO Process (29)
GO Function (8)
GO Component (21)
Homo sapiens
PREY

APPBP2

APP-BP2, HS.84084, PAT1
amyloid beta precursor protein (cytoplasmic tail) binding protein 2
GO Process (3)
GO Function (2)
GO Component (4)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein.

Zheng P, Eastman J, Vande Pol S, Pimplikar SW

In epithelial cells, sorting of membrane proteins to the basolateral surface depends on the presence of a basolateral sorting signal (BaSS) in their cytoplasmic domain. Amyloid precursor protein (APP), a basolateral protein implicated in the pathogenesis of Alzheimer's disease, contains a tyrosine-based BaSS, and mutation of the tyrosine residue results in nonpolarized transport of APP. Here we report identification of ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 08, 1998; 95(25);14745-50 [Pubmed: 9843960]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
APPBP2 APP
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
APP APPBP2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID