CRK
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
BCAR1
Gene Ontology Biological Process
- B cell receptor signaling pathway [ISO]
- G-protein coupled receptor signaling pathway [IDA]
- actin cytoskeleton organization [ISO]
- actin filament organization [ISO]
- antigen receptor-mediated signaling pathway [ISO]
- cell chemotaxis [ISO]
- cell migration [IDA, ISO]
- cellular response to hepatocyte growth factor stimulus [ISO]
- epidermal growth factor receptor signaling pathway [ISO]
- hepatocyte growth factor receptor signaling pathway [ISO]
- insulin receptor signaling pathway [ISO]
- integrin-mediated signaling pathway [ISO]
- neurotrophin TRK receptor signaling pathway [ISO]
- platelet-derived growth factor receptor signaling pathway [IDA]
- positive regulation of cell migration [ISO]
- positive regulation of endothelial cell migration [ISO]
- vascular endothelial growth factor receptor signaling pathway [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Insulin stimulates the tyrosine dephosphorylation of docking protein p130cas (Crk-associated substrate), promoting the switch of the adaptor protein crk from p130cas to newly phosphorylated insulin receptor substrate-1.
The docking protein p130(cas) (Crk-associated substrate) forms a stable complex with the adaptor protein CrkII in a tyrosine-phosphorylation-dependent manner. Insulin-induced tyrosine phosphorylation of insulin receptor substrates results in the redistribution of CrkII between p130(cas) and insulin receptor substrate-1. A decrease in the association between CrkII and p130(cas) in response to insulin stimulation was detected in CHO cells stably expressing insulin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CRK BCAR1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID