BAIT

MXD4

2810410A03Rik, Mad4, bHLHc12
Max dimerization protein 4
GO Process (1)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Mus musculus
PREY

SIN3A

AW553200, Sin3, mKIAA4126, mSin3A
transcriptional regulator, SIN3A (yeast)
GO Process (26)
GO Function (14)
GO Component (10)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation.

Hurlin PJ, Queva C, Koskinen PJ, Steingrimsson E, Ayer DE, Copeland NG, Jenkins NA, Eisenman RN

The basic helix-loop-helix-leucine zipper (bHLHZip) protein Max associates with members of the Myc family, as well as with the related proteins Mad (Mad1) and Mxi1. Whereas both Myc:Max and Mad:Max heterodimers bind related E-box sequences, Myc:Max activates transcription and promotes proliferation while Mad:Max represses transcription and suppresses Myc dependent transformation. Here we report the identification and characterization of two novel ... [more]

EMBO J. Nov. 15, 1995; 14(22);5646-59 [Pubmed: 8521822]

Throughput

  • Low Throughput

Curated By

  • BioGRID