BAIT

CBK1

CaO19.12375, CaO6.3756, CAWG_00395, CA2022, orf19.4909, orf6.3756, CaO19.4909, Contig4-2855_0009, C1_10380C, orf19.12375, IPF9611.2, C1_10380C_B, IPF22379.1, C1_10380C_A
Ser/Thr kinase of cell wall integrity pathway; mutants show abnormal morphology and aggregation; Mob2p associated; required for wild-type hyphal growth and transcriptional regulation of cell-wall-associated genes
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)
PREY

SSD1

CA0317, orf6.1554, CAWG_04840, orf19.3959, C5_04730C, CaO6.1554, IPF18942.2, orf6.1117, C5_04730C_B, CaO19.11441, IPF5791.2, CaO19.3959, orf19.11441, CaO6.1117, Contig4-3081_0004, C5_04730C_A
Protein with role in resistance to host antimicrobial peptides; virulence role in murine infection; functional homolog of S. cerevisiae Ssd1p, which suppresses various mutant phenotypes; constitutively expressed and not cell-cycle regulated
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)

Synthetic Haploinsufficiency

A genetic interaction is inferred when mutations or deletions in separate genes, at least one of which is hemizygous, cause a minimal phenotype alone but result in lethality when combined in the same cell under a given condition.

Publication

A large-scale complex haploinsufficiency-based genetic interaction screen in Candida albicans: analysis of the RAM network during morphogenesis.

Bharucha N, Chabrier-Rosello Y, Xu T, Johnson C, Sobczynski S, Song Q, Dobry CJ, Eckwahl MJ, Anderson CP, Benjamin AJ, Kumar A, Krysan DJ

The morphogenetic transition between yeast and filamentous forms of the human fungal pathogen Candida albicans is regulated by a variety of signaling pathways. How these pathways interact to orchestrate morphogenesis, however, has not been as well characterized. To address this question and to identify genes that interact with the Regulation of Ace2 and Morphogenesis (RAM) pathway during filamentation, we report ... [more]

PLoS Genet. Apr. 01, 2011; 7(4);e1002058 [Pubmed: 22103005]

Throughput

  • High Throughput

Ontology Terms

  • filamentous growth (APO:0000027)

Additional Notes

  • A heterozygous mutant of CBK1, was subjected to transposon-mediated, insertional mutagenesis.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SSD1 CBK1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
SSD1 CBK1
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
2451771
SSD1 CBK1
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
2451828
SSD1 CBK1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID