TLE3
Gene Ontology Biological Process
Gene Ontology Molecular Function
NKX2-2
Gene Ontology Biological Process
- astrocyte differentiation [IMP]
- cell development [IMP]
- cell differentiation [IMP]
- digestive tract development [IMP]
- endocrine pancreas development [IMP]
- negative regulation of neuron differentiation [IDA, IMP]
- nervous system development [IDA]
- neuron fate specification [IGI, IMP]
- oligodendrocyte development [IMP]
- pancreatic A cell fate commitment [IGI, IMP]
- pancreatic PP cell fate commitment [IGI]
- positive regulation of cell differentiation [IMP]
- positive regulation of gene expression [IDA]
- positive regulation of neuron differentiation [IDA, IMP]
- positive regulation of oligodendrocyte differentiation [IGI]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of cell differentiation [IMP]
- regulation of transcription, DNA-templated [IDA]
- smoothened signaling pathway [IDA]
- spinal cord motor neuron differentiation [IMP]
- spinal cord oligodendrocyte cell differentiation [IGI, IMP]
- spinal cord oligodendrocyte cell fate specification [IDA]
- transcription from RNA polymerase II promoter [IDA]
- type B pancreatic cell development [IDA, IMP]
- type B pancreatic cell fate commitment [IMP]
- ventral spinal cord interneuron fate determination [IMP]
Gene Ontology Molecular Function- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin binding [IDA]
- core promoter proximal region DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin binding [IDA]
- core promoter proximal region DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
- intracellular [IDA]
- nucleus [IDA, ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Nkx2.2-repressor activity is sufficient to specify alpha-cells and a small number of beta-cells in the pancreatic islet.
The homeodomain protein Nkx2.2 (Nkx2-2) is a key regulator of pancreatic islet cell specification in mice; Nkx2.2 is essential for the differentiation of all insulin-producing beta-cells and of the majority of glucagon-producing alpha-cells, and, in its absence, these cell types are converted to a ghrelin cell fate. To understand the molecular functions of Nkx2.2 that regulate these early cell-fate decisions ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TLE3 NKX2-2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID