Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Sequential interaction of actin-related proteins 2 and 3 (Arp2/3) complex with neural Wiscott-Aldrich syndrome protein (N-WASP) and cortactin during branched actin filament network formation.

Uruno T, Liu J, Li Y, Smith N, Zhan X

The WASP and cortactin families constitute two distinct classes of Arp2/3 modulators in mammalian cells. Physical and functional interactions among the Arp2/3 complex, VCA (a functional domain of N-WASP), and cortactin were examined under conditions that were with or without actin polymerization. In the absence of actin, cortactin binds significantly weaker to the Arp2/3 complex than VCA. At concentrations of ... [more]

J. Biol. Chem. Jul. 11, 2003; 278(28);26086-93 [Pubmed: 12732638]

Throughput

  • Low Throughput

Additional Notes

  • Arp2/3 complex immunoprecipitated using a GST-tagged VCA peptide derived from human N-WASP

Curated By

  • BioGRID