PREY

TPI1

triose-phosphate isomerase TPI1, L000002326, YDR050C
Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

IN Saccharomyces cerevisiae A GLYCOLYTIC METABOLON IS STABILIZED BY F-ACTIN.

Araiza-Olivera D, Chiquete-Felix N, Rosas-Lemus M, Sampedro JG, Pena A, Mujica A, Uribe-Carvajal S

In the Saccharomyces cerevisiae glycolytic pathway, eleven enzymes catalyze the stepwise conversion of glucose to 2 molecules of ethanol plus 2 CO2 s. In the highly crowded cytoplasm, this pathway would be very inefficient if it were dependent on substrate/enzyme diffusion. Therefore, the existence of a multienzymatic glycolytic complex has been suggested. This complex likely uses the cytoskeleton to stabilize ... [more]

FEBS J. Jun. 14, 2013; 0(0); [Pubmed: 23763840]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TPI1 ACT1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1902BioGRID
1925044
ACT1 TPI1
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High0.163BioGRID
1872850

Curated By

  • BioGRID