PREY

CDKN1B

AA408329, AI843786, Kip1, p27, p27Kip1
cyclin-dependent kinase inhibitor 1B
GO Process (26)
GO Function (7)
GO Component (4)

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

p27Kip1 localization depends on the tumor suppressor protein tuberin.

Rosner M, Freilinger A, Hanneder M, Fujita N, Lubec G, Tsuruo T, Hengstschlaeger M

p27(Kip1) plays an important role in cell cycle regulation by inhibiting cyclin-CDK complex activity in the nucleus. p27(Kip1) is regulated by its concentration as well as by its subcellular localization. Tuberin, encoded by the tuberous sclerosis tumor suppressor gene TSC2, is a potent negative cell cycle regulator. We show herein, that tuberin induces nuclear p27 localization by inhibiting its 14-3-3-mediated ... [more]

Hum. Mol. Genet. Jul. 01, 2007; 16(13);1541-56 [Pubmed: 17470459]

Throughput

  • Low Throughput

Additional Notes

  • interaction positively regulated by phosphorylation of p27Kip1 by Akt

Curated By

  • BioGRID