GSPT2
Gene Ontology Biological Process
Gene Ontology Molecular Function
PABPC1
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- gene silencing by RNA [ISS]
- mRNA metabolic process [TAS]
- mRNA polyadenylation [TAS]
- mRNA splicing, via spliceosome [IC]
- mRNA stabilization [TAS]
- negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [ISS]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [ISS]
- positive regulation of translation [TAS]
- translation [TAS]
- translational initiation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay.
The nonsense-mediated decay (NMD) pathway subjects mRNAs with premature termination codons (PTCs) to rapid decay. The conserved Upf1-3 complex interacts with the eukaryotic translation release factors, eRF3 and eRF1, and triggers NMD when translation termination takes place at a PTC. Contrasting models postulate central roles in PTC-recognition for the exon junction complex in mammals versus the cytoplasmic poly(A)-binding protein (PABP) ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GSPT2 PABPC1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9861 | BioGRID | 2242982 | |
| GSPT2 PABPC1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.987 | BioGRID | 3038314 | |
| PABPC1 GSPT2 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | High | - | BioGRID | - | |
| PABPC1 GSPT2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| GSPT2 PABPC1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 870314 | |
| PABPC1 GSPT2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID