BAIT

CALM1

CALML2, CAMI, CPVT4, DD132, PHKD, caM
calmodulin 1 (phosphorylase kinase, delta)
GO Process (43)
GO Function (11)
GO Component (13)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry.

Berggard T, Arrigoni G, Olsson O, Fex M, Linse S, James P

Calmodulin is an essential Ca2+-binding protein that binds to a variety of targets that carry out critical signaling functions. We describe the proteomic characterization of mouse brain Ca2+-calmodulin-binding proteins that were purified using calmodulin affinity chromatography. Proteins in the eluates from four different affinity chromatography experiments were identified by 1-DE and in-gel digestion followed by LC-MS/MS. Parallel experiments were performed ... [more]

J. Proteome Res. Mar. 01, 2006; 5(3);669-87 [Pubmed: 16512683]

Throughput

  • High Throughput

Curated By

  • BioGRID