BAIT
HTR2B
5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled
GO Process (38)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
- ERK1 and ERK2 cascade [ISO, ISS]
- G-protein coupled receptor internalization [ISO]
- G-protein coupled receptor signaling pathway [ISO, ISS]
- activation of phospholipase C activity [ISO]
- cGMP biosynthetic process [ISO, ISS]
- calcium-mediated signaling [ISO, ISS]
- cardiac muscle hypertrophy [ISO, ISS]
- cellular calcium ion homeostasis [ISO]
- cellular response to temperature stimulus [ISO, ISS]
- embryonic morphogenesis [ISO, ISS]
- heart development [ISO]
- heart morphogenesis [ISO, ISS]
- intestine smooth muscle contraction [ISO, ISS]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of autophagy [ISO, ISS]
- negative regulation of cell death [ISO, ISS]
- neural crest cell differentiation [ISO, ISS]
- neural crest cell migration [ISO, ISS]
- phosphatidylinositol 3-kinase signaling [ISO, ISS]
- phosphorylation [ISO, ISS]
- positive regulation of ERK1 and ERK2 cascade [ISO, ISS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO, ISS]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of Ras GTPase activity [ISO, ISS]
- positive regulation of cell division [ISO, ISS]
- positive regulation of cell proliferation [ISO, ISS]
- positive regulation of cytokine production [ISO, ISS]
- positive regulation of cytokine secretion [ISO, ISS]
- positive regulation of endothelial cell proliferation [ISO, ISS]
- positive regulation of nitric-oxide synthase activity [ISO, ISS]
- positive regulation of phosphatidylinositol biosynthetic process [ISO, ISS]
- protein kinase C signaling [ISO, ISS]
- protein kinase C-activating G-protein coupled receptor signaling pathway [ISO, ISS]
- regulation of behavior [ISO, ISS]
- release of sequestered calcium ion into cytosol [ISO, ISS]
- response to drug [ISO, ISS]
- serotonin receptor signaling pathway [ISO, ISS]
- vasoconstriction [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytoplasm [ISO, ISS]
- intracellular [ISO]
- plasma membrane [IDA, ISO, ISS]
Rattus norvegicus
PREY
AGTR1A
AT1, AT1A, AT1R, Agtr1
angiotensin II receptor, type 1a
GO Process (40)
GO Function (10)
GO Component (15)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IMP, ISO]
- Rho protein signal transduction [ISO]
- activation of Janus kinase activity [IMP]
- aging [IEP]
- angiotensin-activated signaling pathway [ISO]
- blood vessel development [ISO]
- brain renin-angiotensin system [ISO]
- calcium-mediated signaling [ISO]
- cell chemotaxis [ISO]
- cellular response to dexamethasone stimulus [IDA]
- dopamine biosynthetic process [IMP]
- drinking behavior [IMP, ISO]
- heart development [ISO]
- inflammatory response [ISO]
- kidney development [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of smooth muscle cell apoptotic process [ISO]
- phospholipase C-activating G-protein coupled receptor signaling pathway [IDA]
- phospholipase C-activating angiotensin-activated signaling pathway [IDA, IMP, ISO]
- positive regulation of blood pressure [IMP, ISO]
- positive regulation of branching involved in ureteric bud morphogenesis [IMP]
- positive regulation of cell proliferation [IMP, ISO]
- positive regulation of cellular protein metabolic process [ISO]
- positive regulation of cholesterol esterification [ISO]
- positive regulation of cytokine secretion [ISO]
- positive regulation of cytosolic calcium ion concentration [IDA, ISO]
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway [ISO]
- positive regulation of phospholipase A2 activity [ISO]
- positive regulation of receptor recycling [IDA]
- positive regulation of superoxide anion generation [IMP]
- regulation of blood pressure [TAS]
- regulation of pH [IDA]
- regulation of renal output by angiotensin [ISO]
- regulation of smooth muscle cell apoptotic process [ISO]
- regulation of systemic arterial blood pressure by circulatory renin-angiotensin [ISO]
- regulation of vasoconstriction [ISO]
- renin secretion into blood stream [ISO]
- renin-angiotensin regulation of aldosterone production [IMP, ISO]
- response to estrogen [IDA, IEP]
- vasoconstriction [IMP]
Gene Ontology Molecular Function- D1 dopamine receptor binding [IPI]
- G-protein alpha-subunit binding [IMP]
- angiotensin type I receptor activity [IDA, IMP, ISO]
- angiotensin type II receptor activity [ISO]
- bradykinin receptor binding [ISO]
- peptide hormone binding [IMP]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase binding [IPI]
- protein self-association [IMP]
- D1 dopamine receptor binding [IPI]
- G-protein alpha-subunit binding [IMP]
- angiotensin type I receptor activity [IDA, IMP, ISO]
- angiotensin type II receptor activity [ISO]
- bradykinin receptor binding [ISO]
- peptide hormone binding [IMP]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase binding [IPI]
- protein self-association [IMP]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- basolateral plasma membrane [IDA]
- cell [ISO]
- cytoplasm [ISO]
- cytoplasmic vesicle [IDA]
- dendrite [IDA]
- endomembrane system [IDA]
- endosome [ISO]
- integral component of membrane [TAS]
- membrane [IDA]
- organelle outer membrane [IDA]
- plasma membrane [IDA, ISO]
- recycling endosome [IDA]
- vesicle lumen [IDA]
- vesicle membrane [IDA]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Serotonin and angiotensin receptors in cardiac fibroblasts coregulate adrenergic-dependent cardiac hypertrophy.
By mimicking sympathetic stimulation in vivo, we previously reported that mice globally lacking serotonin 5-HT(2B) receptors did not develop isoproterenol-induced left ventricular hypertrophy. However, the exact cardiac cell type(s) expressing 5-HT(2B) receptors (cardiomyocytes versus noncardiomyocytes) involved in pathological heart hypertrophy was never addressed in vivo. We report here that mice expressing the 5-HT(2B) receptor solely in cardiomyocytes, like global 5-HT(2B) ... [more]
Circ. Res. Jan. 02, 2009; 104(1);113-23 [Pubmed: 19023134]
Throughput
- Low Throughput
Ontology Terms
- biological process: cytokine secretion (GO:0050663)
- phenotype: cardiac hypertrophy (MP:0001625)
- tissue: cardiofibroblast (BTO:0003093)
- tissue: myocardium (UBERON:0002349)
Curated By
- BioGRID