BAIT

RAD52

RP11-359B12.2
RAD52 homolog (S. cerevisiae)
GO Process (5)
GO Function (2)
GO Component (2)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Streamline proteomic approach for characterizing protein-protein interaction network in a RAD52 protein complex.

Du Y, Zhou J, Fan J, Shen Z, Chen X

Large-scale identification of protein-protein interactions (PPIs) in functional complexes represents an efficient route to elucidate the regulatory rules of cellular functions. Whereas many methods have been developed to identify the PPIs associated with particular target/bait protein in complexes, little information is available about the interaction relationships among all components in a complex. Here, we have established a strategy of integrating ... [more]

J. Proteome Res. May. 01, 2009; 8(5);2211-7 [Pubmed: 19338310]

Throughput

  • Low Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007)

Additional Notes

  • Exogenous expression of bait

Curated By

  • BioGRID