An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Streamline proteomic approach for characterizing protein-protein interaction network in a RAD52 protein complex.

Du Y, Zhou J, Fan J, Shen Z, Chen X

Large-scale identification of protein-protein interactions (PPIs) in functional complexes represents an efficient route to elucidate the regulatory rules of cellular functions. Whereas many methods have been developed to identify the PPIs associated with particular target/bait protein in complexes, little information is available about the interaction relationships among all components in a complex. Here, we have established a strategy of integrating ... [more]

J. Proteome Res. May. 01, 2009; 8(5);2211-7 [Pubmed: 19338310]

Throughput

Low Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007)

Additional Notes

Exogenous expression of bait

Curated By

BioGRID

Interaction Summary

RAD52

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

ALDOA

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [TAS]cytoskeletal protein binding [IDA]fructose binding [IDA]fructose-bisphosphate aldolase activity [IDA]identical protein binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]tubulin binding [TAS]