BAIT
RAD52
RP11-359B12.2
RAD52 homolog (S. cerevisiae)
GO Process (5)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [TAS]
- nucleus [TAS]
Homo sapiens
PREY
SEC13
D3S1231E, SEC13L1, SEC13R, npp-20
SEC13 homolog (S. cerevisiae)
GO Process (10)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
- COPII vesicle coating [TAS]
- ER to Golgi vesicle-mediated transport [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cellular protein metabolic process [TAS]
- intracellular protein transport [NAS]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Streamline proteomic approach for characterizing protein-protein interaction network in a RAD52 protein complex.
Large-scale identification of protein-protein interactions (PPIs) in functional complexes represents an efficient route to elucidate the regulatory rules of cellular functions. Whereas many methods have been developed to identify the PPIs associated with particular target/bait protein in complexes, little information is available about the interaction relationships among all components in a complex. Here, we have established a strategy of integrating ... [more]
J. Proteome Res. May. 01, 2009; 8(5);2211-7 [Pubmed: 19338310]
Throughput
- Low Throughput
Ontology Terms
- hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Curated By
- BioGRID