An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Streamline proteomic approach for characterizing protein-protein interaction network in a RAD52 protein complex.

Du Y, Zhou J, Fan J, Shen Z, Chen X

Large-scale identification of protein-protein interactions (PPIs) in functional complexes represents an efficient route to elucidate the regulatory rules of cellular functions. Whereas many methods have been developed to identify the PPIs associated with particular target/bait protein in complexes, little information is available about the interaction relationships among all components in a complex. Here, we have established a strategy of integrating ... [more]

J. Proteome Res. May. 01, 2009; 8(5);2211-7 [Pubmed: 19338310]

Throughput

Low Throughput

Ontology Terms

hek-293 cell (BTO:0000007)

Additional Notes

Exogenous expression of bait

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAD52 HSPA4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Du Y (2009)	Low	-	BioGRID	870573

Curated By

BioGRID

Interaction Summary

RAD52

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

HSPA4