BAIT

SMAD4

DPC4, JIP, MADH4, MYHRS
SMAD family member 4
GO Process (24)
GO Function (14)
GO Component (9)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Novel function of androgen receptor-associated protein 55/Hic-5 as a negative regulator of Smad3 signaling.

Wang H, Song K, Sponseller TL, Danielpour D

Androgen receptor-associated protein 55 (ARA55/Hic-5) belongs to the LIM protein superfamily and is featured by three or four N-terminal LD motifs and four C-terminal zinc finger-like LIM domains. Both LD motifs and LIM domains can serve as protein-protein interaction interfaces. Recently, we found that enforced expression of ARA55 inhibits transforming growth factor-beta-mediated up-regulation of Smad binding element-luciferase reporter activity in ... [more]

J. Biol. Chem. Feb. 18, 2005; 280(7);5154-62 [Pubmed: 15561701]

Throughput

  • Low Throughput

Curated By

  • BioGRID