An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells.

Roux KJ, Kim DI, Raida M, Burke B

We have developed a new technique for proximity-dependent labeling of proteins in eukaryotic cells. Named BioID for proximity-dependent biotin identification, this approach is based on fusion of a promiscuous Escherichia coli biotin protein ligase to a targeting protein. BioID features proximity-dependent biotinylation of proteins that are near-neighbors of the fusion protein. Biotinylated proteins may be isolated by affinity capture and ... [more]

J. Cell Biol. Mar. 19, 2012; 196(6);801-10 [Pubmed: 22412018]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LMNA PDAP1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2022)	High	-	BioGRID	3434735

Curated By

BioGRID

Interaction Summary

LMNA

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]structural molecule activity [TAS]

Gene Ontology Cellular Component

PDAP1