BAIT

KCNK9

K2p9.1, KT3.2, TASK-3, TASK3
potassium channel, subfamily K, member 9
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

YWHAB

GW128, HEL-S-1, HS1, KCIP-1, YWHAA, RP1-148E22.1
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Potential use of potassium efflux-deficient yeast for studying trafficking signals and potassium channel functions.

Bernstein JD, Okamoto Y, Kim M, Shikano S

The activity of potassium (K(+)) channels critically depends on their density on the cell surface membrane, which is regulated by dynamic protein-protein interactions that often involve distinct trafficking signals on the cargo proteins. In this paper we explored the possibility of utilizing the Saccharomyces cerevisiae strain B31 for identification of the signal motifs that regulate surface expression of membrane proteins ... [more]

FEBS Open Bio Jun. 19, 2013; 3(0);196-203 [Pubmed: 23772394]

Throughput

  • Low Throughput

Curated By

  • BioGRID