An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Brain-Specific Angiogenesis Inhibitor-1 Signaling, Regulation and Enrichment in the Postsynaptic Density.

Stephenson JR, Paavola KJ, Schaefer SA, Kaur B, Van Meir EG, Hall RA

Brain-specific angiogenesis inhibitor-1 (BAI1) is an adhesion G protein-coupled receptor (GPCR) that has been studied primarily for its anti-angiogenic and anti-tumorigenic properties. We found that over-expression of BAI1 results in activation of the Rho pathway via a Galpha12/13-dependent mechanism, with truncation of the BAI1 N-terminus (NT) resulting in a dramatic enhancement in receptor signaling. This constitutive activity of the truncated ... [more]

J. Biol. Chem. Jun. 19, 2013; 0(0); [Pubmed: 23782696]

Throughput

Low Throughput

Additional Notes

Association between the NT and 7TM regions of BAI1

Curated By

BioGRID

Interaction Summary

BAI1

Gene Ontology Biological Process

Gene Ontology Cellular Component